ABSTRACT

The development of the moss protonema and subsequent differentiation of buds are regulated by a variety of environmental factors such as light intensity, photoperiod, temperature, and humidity. Under experimental conditions, the moss protonema behaves abnormally when subjected to certain “stress conditions” such as low light intensity and submerged culture conditions. Rapid advances in the use of techniques such as high performance liquid chromatography, capillary gas chromatography, and mass spectrometry have resulted in the development of new methods for the separation, identification, and quantitative assay of plant hormones. The accumulation of a substance within the cell is the combined effect of its influx and efflux. A symplastic exchange of substances between adjacent cells also must take place because tip cells of the caulonema are required to maintain the filaments in a stable state of differentiation.