ABSTRACT
Lignocellulosic biomass has been considered as renewable feedstock for liquid biofuels generations. Lignocellulosic residues are inedible, inexpensive, and immensely available in nature which makes it attractive alternative toward belittling fossil fuels reserves. Liquid biofuels generations from lignocellulosic residues have positive socioeconomic impacts likely reduction in agricultural land usage, water utilization, and greenhouse gases emissions. Liquid biofuels have higher octane and cetane numbers, which makes it better fuels with higher combustion speed and ignition quality. Naive microorganisms involve in lignocellulosic bioconversions toward liquid biofuels, however, processes are limited due to lower titer, molar yield, and productivity. It seems naive microorganisms are inefficient to degrade lignocellulosic biomass into accessible monomers. Competing metabolic pathways in native microbes are redirecting flux toward by-products accumulations. Hence, current chapter focuses on metabolic engineering and synthetic biology approaches to resolve these bottlenecks by knocking out competing pathways and introducing novel pathways for ameliorating liquid biofuels generations.
