ABSTRACT
Biohydrogen production from dark fermentation (DF) of lignocellulosic biomass has been gaining much attention over the past years. This chapter provides an insight of the DF process and the different parameters that influence biohydrogen production from lignocellulosic biomass such as pH and temperature, among others. The chapter also reviews some of the factors that have been commonly reported to inhibit the DF process while also outlining some of the strategies that have been studied for enhancing dark fermentative biohydrogen production such as inoculum and substrates pretreatment. This chapter also provides an overview of the potential use of the DF effluent as substrates for further bioenergy production through coupling of the DF process with other techniques such as anaerobic digestion, photofermentation, or bioelectrochemical systems. Finally, the chapter outlines some of the attempts made toward the upscaling of the DF process.
