ABSTRACT
One of the more important variations is the ability or the lack of it for antisera to recognize morphine glucuronide. Although some investigators felt that some degradation does occur, it was admitted that there are little hard data to support this. Some of the experiences may vary due to different conditions. A solid phase method was also described. In this approach, the antisera is coated onto polystyrene cups, which may be frozen and stored until use. The sample containing the drug and the marker are added, the cups incubated, the supernatant removed, and the contents counted by dissolution of the cups in the scintillation solvent. The presence of lysozymes and complement proteins in serum requires that these be removed before the analysis. Crab shells may be used to remove lysozyme, but denaturing by heating is required for the proteins. There is general agreement about the variations in specificity from animal to animal and bleeding to bleeding.
