ABSTRACT
The concept that regulation of immune responses (Ir) occurs through cellular interactions restricted by recognition of “self”-determinants is an established tenet of immunology. One in vitro correlate of the “self”-recognition principle is the autologous mixed lymphocyte reaction (AMLR). The AMLR is the proliferation of T cells when they are appropriately cultured with autologous non-T cells in the absence of added antigen or lectin. Since the proliferation is induced by autologous cells, the AMLR appears to be an in vitro manifestation of “self”-recognition. In general, responding cells consisted of E rosette-positive populations while stimulating populations consisted of E rosette-negative cells. Stimulator cells were further fractionated into adherent cell-enriched populations by 2, 1 hr adherences onto polystyrene petri dishes in the presence of 10% nonheat-inactivated autologous serum. Thus, a decision concerning the reactivity of cells responsive in the AMLR to alloantigens awaits studies using established T cell clones.
