ABSTRACT
A list of labeled tracers, along with the method of labeling and nature of biomedical studies. Many tracers are commonly used in nuclear medicine as radiopharmaceuticals labeled with different isotopes. In order to understand the chemistry of iodination of small molecules and proteins, it is important to know the reactions of iodine with the solvent, the reactions of active species formed during oxidation with molecules undergoing radioiodination, and the side-reactions of oxidizing and reducing agents with susceptible groups. In general radioiodination of a protein thus leads to the formation of a heterogeneous mixture of tyrosyl and histidyl and some tryptophan derivatives. The chloramine-T method is most widely used for radioiodination of a large number of proteins for obtaining higher specific activity. Radioiodination with the enzyme incorporated in the bead form provides a more convenient and gentle procedure. Personnel carrying out radioiodinations should put on disposable gowns, two pairs of gloves, masks, caps, and shoe covers.
