ABSTRACT
The “closed chamber” technique has definite advantages to the “open” technique for the characterization of mononuclear cells in situ in various tissue infiltrates. The technique is a valuable tool for the further characterization of mononuclear cells in infiltrates in various dermatoses, and the results should contribute to a better understanding of the disease process. Advances in immunopathology have demonstrated a distinct heterogeneity in mononuclear cells in various cell infiltrates. Erythrocytes are the most commonly used particles. The use of membrane receptors for the classification and differentiation of mononuclear cells using erythrocytes as indicator cells is presently widely accepted and can be applied in studies with tissue sections. The ratio of the cells in blood to cells in tissue infiltrates can be an important indication of selective accumulation of lymphocytes in various diseases. When the coverglass around the tissue is free of erythrocytes, the degree of hemadsorption is recorded microscopically.
