ABSTRACT
Available resources, frequency of isolation, and need determine how far to proceed with isolation and identification of glucose nonfermenting Gram-negative rods in the clinical laboratory. A system should be adopted for isolation, recognition, and systematic identification of glucose nonfermenting Gram-negative rods. A selective-differential medium should be used with one of the nonselective media for primary isolation of glucose nonfermenting Gram-negative rods. The optimum growth medium and the optimum temperature for growth may not be the ideal environment for synthesis of flagella and detection of motility. Many glucose nonfermenting Gram-negative rods have been characterized by their ability to grow at the expense of organic compounds, using them as the sole sources of carbon and energy in mineral-basemedia containing ammonium ions as the sole source of nitrogen. Extracellular deoxyribonuclease produced by glucose nonfermenting Gram-negative rods can be detected by inoculation of an agar medium containing deoxyribonucleic acid.
