ABSTRACT
This chapter aims to describe in detail the basic make up and variations in construction of continuous cultures that the researcher can use. Inflow medium is routinely added to the vessel in a drop wise fashion. In addition, it is necessary to prevent contamination of the inflow medium from organisms in the culture vessel. Many variations on the simplest Pasteur pipette exist. The inflow gas is sterilized by passage through appropriate membrane filters, cotton, or glass wool. The inflow gas is sterilized by passage through appropriate membrane filters, cotton, or glass wool. The former requires a drive motor above the culture with an entry port for the drive rod. Once the apparatus has been set up and is found to be working satisfactorily, and before inoculation, the author routinely fills the culture vessel with a small fraction of growth medium, starts the stirrer and air pump.
