ABSTRACT
This investigation deals with the influence of melafen (melamine salt of bis(oximethyl) phosphinic acid) on the structural properties of membrane-bounded proteins. Melafen is applied in agriculture as a strong plant growth regulator. Melafen was used as aqua solutions, at a wide concentration's region at all our investigations. The erythrocyte's ghost that was isolated from blood erythrocytes were used as the experimental objects. The melafen interactions with membrane-bounded proteins at the erythrocyte's ghost were tested by the differential scanning microcalorimetry (DSC) method. The application of DSC allowed us to receive the structural data of protein microdomains organization at erythrocyte's ghost membranes. We did not reveal any noticeable structural changes of the protein's microdomain organization at erythrocyte ghost membranes under the large concentrations of melafen. The melafen concentrations, which are presented at our experiments, were so much bigger, than concentrations of melafen, which are used at crop production. The melafen actions to the protein-lipid interrelationships were tested by registrations of the microviscosity of isolated native 102erythrocytes. The using of electron spin resonance method (ESR) with the introduction of spin-labeled probes to erythrocytes allowed us to receive some structural data of protein-lipid organization at the different depths of erythrocyte's membranes (2–4 Å; 4–8 Å). The application of the spectral method for registration of hemolysis degree, allowed us to receive some data about melafen influences to membrane resistance under the bed environment, when the hypo- and hyper-osmolarity existed. We concluded that melafen has not any destruction's influences to the membranes bounded proteins under the concentrations that activate the plant growth. But melafen had essential influencing to the structural and functional properties of membrane-bounded proteins when we used the bigger concentrations.
