ABSTRACT
The fibrin sealant emanates from the unique complex structure of the native soluble plasma protein fibrinogen comprising an intricate arrangement of three pairs of polypeptide chains with two connecting three regions of more compact, complicated trinodular configurations. The autologous native cryoprecipitated fibrinogen concentrates, produced under blood-bank standards, have yet to be assessed for effectiveness during in vivo tissue healing in terms of tissue strength and elasticity in order to complete restoration of biomechanical integrity. It is to this restoration of tissue healing, using cryoprecipitated native fibrinogen, that this presentation is directed. It illustrates a fibrin sealant reference standard compared to routine suture closure in an in vivo animal model. The fibrin-sealant reference standard of this restricted presentation with specified test dimensions is intended to serve as a basis for the numerous other chemically variant precipitated fibrinogens, with further considerations of variables of adjustments in pH, ion concentrations, and imposed means of viral inactivation.
