ABSTRACT
In bone tissue engineering, the biodegradable scaffolds will play a role as a temporary template for new bones tissues. Freezing freeze-drying technique involves creating a solution using polymers and solvent, quickly freezing the solution to lock liquid state structure and then remove the solvent by freeze drying. This chapter focuses on the fabrication and evaluation of polycaprolactone (PCL)/chitosan-based scaffolds using a freeze-drying technique. Bone is a complex and highly specialized form of connective tissues pertaining to the formation of the skeleton of the body. Mechanical properties of the scaffold should match the host bone properties and have proper load transferred to support the harboring of new bone tissues and support and also the mechanical integrity of the implanted area. Nano-sized hydroxyapatite and tetracycline HCL were incorporated into the scaffolds to study their characteristics, release and biocompatibility with human osteoblast cells via in vitro studies.
