ABSTRACT

An adjustable electronic aerosol atomization system was designed to produce different airflow rates, in which it can be used to disperse small volume of cells-alginate suspension in microlitres and to generate narrow range of microcapsules ranging from 80 to 360 µm. The electronic aerosol atomization system was set to extrude microdroplets of cells-alginate at an air flow rate of 0.3 l/min and an extrusion rate of 20 µl/min. During the experiment, the aerosol system dispersed the microdroplets of cell-alginate from the aperture of the needle, and the microdroplets dropped into the petri dish followed by polymerization in calcium alginate solution for approximately 10 min. Aerosol atomization technique is an efficient technique to microencapsulate cells in calcium alginate leading to the growth of microtissues within 16 days.