ABSTRACT
Sample preparation has always been an important issue in any analytical procedure for the determination of dye residues in various samples. With increasing demand for the confirmation of dyes present in various samples especially in food, ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) based methods have been widely used more frequently. Due to their high sensitivity and selectivity, UPLC-MS based methods provide the capability of multicomponent validation and quantification. Many harmful additives such as synthetic dyes were added into feeds, and their metabolites and harmful residues remain in animal body and eventually turn into edible food products. UPLC system was coupled with an electrospray ionization triple-stage quadrupole mass spectrometer operated in a positive-ion mode for the identification and quantification of Rodamine B. The eluate was electrosprayed, ionized, and monitored by MS–MS detection in the multiple reaction mode using positive electrospray ionization.
