ABSTRACT
Bioanalytical methods have traditionally been used in a variety of disciplines, including various diagnostic examinations in medicine, DNA structure research and its modifications in genetics, and more. It is also widely used in the field of food analysis, especially in so-called screening tests. Bioanalytical methods have two types of concepts. The first concept has alternative approaches to the analysis of “biomolecules.” The second concept interprets the term as a set of techniques utilizing biological molecules to obtain biospecific interaction that allow qualitative or quantitative determination of the analyte(s). From bioanalytical methods, the front of the technique is based on a specific reaction between antibodies and antigens, and the labeling of one of the reactants can be used to detect the reaction. The simplest immunochemical methods utilize a secondary phenomenon that under certain conditions accompanies the formation of an immunocomplex. The immobilized antigen reacts directly with an excess of the antibody-enzyme conjugate.
