ABSTRACT
The BacMam system consists of the modified insect viruses (baculoviruses) as vehicles to efficiently deliver and express foreign genes into mammalian cells. The technique can be mainly developed using Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and nowadays widely applied to large-scale recombinant protein production with appropriate modification, high-throughput screening platform for cell based assay and delivery of large size gene. Bombyx mori nucleopolyhedrovirus (BmNPV) is another baculovirus that infects silkworm. Silkworm is often employed as a rapid and cost-effective approach for recombinant baculovirus generation. This chapter discusses the recent progress of the investigation of the BacMam system using silkworm baculrovirus, which is unique but promises to be widely applicable. Successful experimental procedures are highlighted in detail, which provides the key elements for the strategy and the issues need to be overcome. Further the direction for the future development of this technique including possible application to scientific and industrial fields are proposed by showing reported examples with the AcMNPV-mediated BacMam system.
