ABSTRACT
A diurnal rhythm of volatile biosynthesis and emissions have also been observed in the flowers and leaves of some plant species. The biosynthesis and emission of volatiles can also be influenced by a complex interplay between environmental/abiotic and biotic factors. Differential expression analysis is perhaps the most important statistical approach in many transcriptome analysis studies. Among the specialized compounds responsible for the desired properties of such plant tissues are low-molecular-weight lipophilic compounds with high vapor pressures, called plant volatiles. In the most generalized scenario, such as the case of a nonmodel plant producing unique volatile(s) of interest but with little or no background information available, volatiles and transcriptomes of select tissues or organs, and at various developmental stages, can first be profiled. TF overexpression may accelerate the discovery of volatile pathway genes by activating multiple candidate genes simultaneously and bypassing the need for more complex analysis that takes into account the spatial and temporal variation in volatile gene expression.
