ISOLATION OF β-SITOSTEROL FROM Crotalaria longipes WIGHT & ARN: PHARMACOLOGICAL USES

The aerial part of Crotalaria longipes of family Fabaceae was subjected to isolation and identification of chemical constituents. The extract was purified and isolated by column chromatograph and thin layer chromatography. Then the isolated compound was subjected to UV spectrum, FTIR for recognition of functional groups and ¹HNMR and ¹³CNMR for classification of protons and carbon atoms. ESI–MS was done to identify the molecular weight of the isolated compound. From the spectra obtained from FTIR, ¹HNMR, ¹³CNMR, and ESI–MS, the isolated compound was found to be β-sitosterol.