ABSTRACT

Spontaneous spawns were collected in captivity but both egg quality and quantity were variable. Thus, stripping females and artificial insemination were recommended for industrial regular production, requiring a good knowledge of biology and management of turbot gametes. In the female, this includes the settlement of a stripping protocol preventing ova overripening and increasing larvae production by 300% [3] but also the availability of analytical tools such as pH measurements of ovarian fluid used to estimate ova quality [4]. In the male, different features of sperm biology such as motility characteristics, energetic metabolism, spermatozoon morphology, seminal fluid composition and changes with time of sperm quality were also described [5].