ABSTRACT
Lipases are predominantly significant enzyme owing to the fact that they precisely hydrolyze acyl glycerol, greases and oils, which is one of the great interests for diverse industrial applications. Lipase producing bacterial strains were isolated from saline soil of costal banks of Bhavnagar, Gujarat, India by employing enrichment culture techniques. Tributyrin oil and minimal salt containing media was employed for isolation and sub-culturing bacteria for primary screening. All isolated bacterial strains were screened by the ratio of the lipolytic halo radius and the colonies radius. The strain having the maximum ratio was recognized morphologically, microscopically and on gene level by sequencing of 16S rRNA gene. The most productive strain produced lipase in olive oil containing complex media and the crude lipase preparation was capable of hydrolyzing p-nitrophenyl palmitate over a range of wide pH. The crude lipase presented maximal activity (6.16 ± 0.17 U mL-1 min-1) at pH of 9.0. Based on the outcomes of current study, lipase of isolated bacteria is a possible alkaline lipase and an applicant for industrial claims such as laundry, leather, detergent and fine chemical industries.
