ABSTRACT
Diabetes can cause biological structural changes and functional interruptions, which have drawn much attention. Optical imaging techniques have the advantage of high resolution and low invasiveness, and thus have been widely used for disease research. However, the turbidity of tissue prevents light from penetrating, leading to a limitation of visualization. Fortunately, the novel tissue optical clearing technique can reduce the light scattering of tissue and establish a window for light to obtain information on disease-mediated molecular, cellular, and vascular changes. This chapter firstly introduces how diabetes changes the distribution of collagen in the skin. Then, it will discuss how optical clearing skin windows and skull windows greatly contribute to the observation of diabetes-induced vascular dysfunction. In addition, this chapter will introduce how the tissue optical clearing technique allows confocal microscopy to visualize monocytes/macrophages in diabetic mice.
