ABSTRACT
Raman spectroscopy is one of the most powerful techniques in biomedical research. It is a pivotal tool for in vivo biological sample studies. However, strong elastic scattering decreases the intensity of light propagation into deep tissues. The optical clearing of tissues has been of great interest owing to its great potential in enhancing the capabilities of noninvasive light penetrate. Therefore, a detailed discussion on the use of Raman spectroscopy combined with optical clearing will be reported. This chapter reviews the advantages and disadvantages of Raman spectroscopy for in vivo studies, especially the in vivo application of a portable Raman system.
