ABSTRACT
This chapter describes the advance of gene editing adopted in sorghum by highlighting the few studies completed. A study of the successful application of gene editing in sorghum for protein improvement is described in detail, and the future prospects for CRISPR/Cas9-mediated gene editing in sorghum genetic improvement and agricultural productivity are addressed. We also summarized the screening of stably edited lines, the measurement of protein digestibility and lysine content, and the introgression of high lysine/high digestibility into elite varieties for sorghum breeding. The study described in detail here to improve sorghum digestibility and lysine content by CRISPR/Cas9-mediated k1C gene family editing both highlights the technological advances of the technique but shows its immediate applicability to crop improvement. Sorghum is a drought-tolerant C4 plant, which can be grown in arid and semi-arid regions. The CRISPR/Cas9 strategies in sorghum focus on editing genes individually.
