ABSTRACT
The majority of the reports for solanaceous species represent the creation of indels to disrupt gene expression to understand function. The work presented demonstrated that application of gene editing was effective at modification of traits such as plant architecture, disease resistance, fruit and potato tuber characteristics. Since those first reports, gene editing to create indels to disrupt gene expression in tomato has become routine and utilized for biological studies. For instance, there is a multiplex toolbox available for plants that provide a protocol and reagents to assemble genome editing constructs for dicots and monocots. With recent reports of increases in obesity and diabetes, namely type II, the focus of potato tuber quality improvement has moved toward modifications that would increase the content of less digestible resistant starch, which is primarily composed of amylose and long-unbranched amylopectin. The greatest number of reports by far of gene editing in solanaceous species is through the CRISPR/Cas9 approach to generate indels.
