ABSTRACT

This chapter discusses delivery options, cell type or plant explant, and the potential benefits of using morphogenic genes. Regenerability can be described as the plant cell's ability to switch toward a regenerative pathway resulting in the production of a full fertile plant capable of transmitting the intended genome modification to its progenies. Current delivery options for genome editing reagents can be divided into three main categories: physical, chemical, and biological. One additional advantage of this delivery system is that multiple genome editing components can be co-delivered on separate plasmids, simplifying vector design and assembly. In the past, focus on viral replicons centered mainly on production of recombinant proteins in plants. Continued development of such in planta strategies have great potential for simplifying and accelerating genome editing and may prove useful to a wide range of crops. Genes that control the organized spatiotemporal development of plant embryos, meristems, tissue, and organs have been cloned and characterized since the late 1990s.