ABSTRACT
Upstream processing is an important aspect of any production industries, especially industries dealing with microbial interaction. Pullulan is an exopolysaccharide produced by the yeast-like fungus A. pullulans. The cultures are stirred continuously for providing aeration, and temperature is maintained at 30 C. The maximal culture growth is obtained at 75 H, and pullulan is produced at 100 H. In every method of pullulan production, it is necessary to maintain several culture conditions such as carbon source, nitrogen source, pH, temperature, and other factors such as minerals, vitamins, aeration, agitation, etc. Similarly, when using corn syrup as the carbon source and yeast extract as the nitrogen source, at low pH of 2, pullulan production was very low both in presence and absence of yeast extract. Several factors affecting pullulan production have been discussed, and it is now proposed that various sources and strains require different optimal conditions for pullulan production.
