ABSTRACT

Recombinant proteins are novel proteins produced through DNA technology by the joining of genetic sequences from heterologous sources. Since the first successful DNA cloning experiments in 1973, research has exploded in molecular biology, oligonucleotide synthesis, identification and isolation of enzymes that cleave and join DNA, characterization of bacterial plasmids, and gene expression [31,63]. These areas are now known collectively as the field of biotechnology. In 1976, this research lead to the production of the first recombinant protein, the neurotransmitter human somatostatin [23]. This great achievement was followed shortly by the first commercially successful recombinant protein, human insulin [18].