Chromatography is one of the best research tools for the biosciences. It makes it possible to analyze biomolecules and to obtain the most valuable and important information on them, such as their chemical, physicochemical, and biochemical properties. At the same time, it affords complete separation of components. Therefore, the more sophisticated the chromatographic mode, the higher the quality of the information obtained. For example, affinity chromatography reveals the nature of the active site of enzymes; gel permeation chromatography discloses the size of biomolecules. Moreover, development of high-performance liquid chromatography (HPLC) has greatly enhanced the utility of chromatography by improving speed, sensitivity, reproducibility, and accuracy.