ABSTRACT

A variety of life processes are dependent on the specific biological recognition of macromolecules. Modem separation techniques, such as chromatography [1], electrophoresis [2-4], and centrifugation [5], are widely used for the analysis of specific affinities of biological molecules including proteins, nucleic acids, and sugars. These applications are based on the change in the physicochemical properties of interacting molecules as the result of complex formation. The complex may have properties that are different from those of their component molecules with respect to molecular weight, electrical charge, or hydrodynamic properties, thus permitting its separation from the component molecules.