ABSTRACT
The sequence of events leading from protoplast to normal cell, capable of reproduction, has been referred in the literature as either protoplast regeneration or protoplast reversion. Investigation into the regeneration of fungal protoplasts provides information in several directions. It permits assessment of the repair capacity of the cell, in other words, of the extent to which fungal cell impairment still can be repaired. The regeneration of fungal protoplasts is not only a unique biological phenomenon but also a useful tool for cytological, biochemical, and genetic studies of these organisms. The protoplast state of a cell causes considerable interference with the normal sequence of cell cycle events. A problem of utmost interest in the development of fused protoplasts is the interaction of nuclei. The morphology of protoplast growth and consequently the morphology of reversion depend on the relationship between an increase in cytoplasmic volume and the rate of wall regeneration.
