ABSTRACT
High-performance liquid chromatography (HPLC) is increasingly becoming the premier choice for analyzing antibiotics and anti-infective agents, according to regulatory agencies and compendia. The advantages of HPLC include simplicity, accuracy, precision, versatility, and, most importantly, selectivity, especially to distinguish between constituents of a multicomponent system. Columns of different polarity can be used to analyze a compound, thus enabling many interfering substances, such as those found in biological matrices, to be reduced or eliminated. HPLC is versatile enough to permit several methods of analysis to be developed starting with almost any type of separating column.
