ABSTRACT
The recovery of products from fermentor broths is both difficult and expensive; therefore, downstream processing has long been regarded as the critical factor in the commercial development of biotechnology. Affinity chromatography is undoubtedly the most powerful technique in enzyme purification and the vast number of published papers witness its success in the laboratory. In affinity chromatography, as with other adsorption techniques, a large interfacial surface area between adsorbent and mobile liquid phases is desirable. Triazine dyes comprise chromophores of three main groups: anthraquinone, azo, and phthalocyanine, linked either to a highly reactive dichlorotriazinyl group or to a less reactive monochlorotriazinyl group. The cost of an affinity adsorbent is dependent not only on the type of matrix and ligand employed, but also on the activation method used for ligand immobilization. Safety requirements laid down by the regulatory authorities, and the necessity for the manufacturer to make its process as economical as possible, require regular maintenance of the chromatographic materials.
