ABSTRACT
As one of the methods to control soilborne plant diseases, biological control has received attention mainly as an alternative to use of fungicides or bactericides, with the increased public awareness of the need to protect our environment. This chapter describes characteristics of Bacillus subtilis NB22 in relation to suppressive mechanisms. It demonstrates some examples of this bacterium in suppression of various plant pathogens both in vitro and in vivo. The chapter introduces basic research on the biochemical and genetic analyses on this bacterium. No foam separation and concentration of peptidolipidic substances in a jar fermentor has yet been reported. With the advances in bioreactor-based biotechnological processes, it has become important to develop methods for the isolation and purification of products from cultures or cells that are suitable for large-scale application. Bacillus subtilis NB22 grown in L medium was mutated to obtain iturin-nonproducing mutants.
