ABSTRACT
A stable submicron amphotericin B (AmB) sterile emulsion was prepared using a combination of following three emulsifiers: phospholipids, poloxamer and sodium deoxycholate. The overall results indicate that in murine candidiasis, treatment with AmB submicron emulsion was more effective than Fungizone®, suggesting potential therapeutic application. The present data, using a murine cryptococcosis model, clearly suggest that the brain was more accessible to AmB from the micellar solution than from the emulsion. The difference in the observed survival data, as compared to the survival data of the mice treated with AmB emulsion, was also statistically significant. A control group of 10 infected mice treated with saline instead of AmB was included. The number of surviving animals in each group was recorded daily over a period of 45 days. Male albino BALB/c mice weighing 20 g each were injected through the tail vein with various doses of AmB either as Fungizone® or as various emulsion formulations at different concentrations.
