ABSTRACT
Several reagents have been employed to effect the condensation of two 5'-nucleotides in syntheses of dinucleoside polyphosphates, and particular attention has focused on carbonyldi(azoles) and water-soluble carbodiimides. While most syntheses have focused attention on symmetrical dinucleoside polyphosphates, the condensation methods described lend themselves rather nicely to the preparation of asymmetrical species containing two different nucleosides. The state of synthesis of analogues of dinucleoside polyphosphates appears to be in good shape. The stereochemical control of a-thiophosphate analogues appears both to be under good control and to be capable of further developments for nucleotide work in general. The state of synthesis of analogues of dinucleoside polyphosphates appears to be in good shape. In terms of their biological uses, much has been learned about the mechanisms of the major enzymes involved in the synthesis and degradation of dinucleoside polyphosphates through the application of analogues.
