ABSTRACT
A number of effects have been reported for dinucleoside polyphosphates on several biological systems. There are several reports describing the inhibition of some kinases and other enzymes by NpnN’s. There are some general rules referring to the bisubstrate or transition state analogues that are also applicable to NpnN’s. There are multiple enzymes transforming one substrate, or catalyzing reactions between one substrate of non-nucleotide nature and one nucleotide base, one nucleoside, one nucleotide, or one polynucleotide. There are several reports describing activation effects for Npn N’s. There are four known cases in which NpnN’s may act as acceptors for enzymes catalyzing the polymerization of nucleotide derivatives. Binding experiments allow the study of the interaction of ligands with proteins irrespective of whether the biological role of the protein is known. In developing rat brain neurons, the disappearance of DNA polymerase activity occurring around birth is paralleled by the disappearance of the Ap4 A-binding activity.
