ABSTRACT
Fast Atom Bombardment (FAB) is a mass spectrometric (MS) ionization technique which is particularly suited for the examination of biomolecules such as carbohydrates. Fast Atom Bombardment (FAB) is a mass spectrometric (MS) ionization technique which is particularly suited for the examination of biomolecules such as carbohydrates. The individual signals in a cluster will be observed when the instrumental resolution is set to resolve nominal masses. A good example of where a modification constitutes an improvement over traditional protocols is the peracetylation reaction. Permethylation techniques are ubiquitous in carbohydrate analysis and are almost always based on Hakomori’s classical method with a few notable exceptions. The way in which a sample is analyzed depends on the nature of the compound, how it has been prepared, and what sort of data is required from it. The abundance of fragment ions can sometimes be enhanced by the use of collision gases.
