ABSTRACT
This chapter reports the map locations for and provides molecular comparisons of the members of two duplicate loci present in the soybean genome: sle, a gene that encodes a late embryogenic protein. Comparisons of the nuclear genome of a variety of organisms has indicated that major changes in the amount of nuclear DNA and in the structure of chromosomes have occurred during evolution. Mapping and molecular characterization of additional duplicate loci will aid in our understanding of the relative contribution of these processes to the evolution of the soybean genome, and provide important information on the genomic structure of this agronomically important crop. Consecutive sub-fragments ranging in size from 0.42 kb were isolated from each A071 clone. Highly repetitive sequences were identified by preparing dot blots containing each sub-fragment and hybridizing with labelled soybean genomic DNA under high stringency conditions.
