ABSTRACT
Viability of cryopreserved blastulae in liquid nitrogen (–196°C) of the sea urchin Strongylocentrotus intermedius was investigated. Abnormalities of post-thaw development are described. A few (0.1–0.2%) of cryopreserved blastulae of S. intermedius passed through all stages of larval development and reached metamorphosis. Young urchins (age 352 days, 8–30 mm) raised from frozen blastulae attained sexual maturity and produced sound offsprings. Development of embryos of the second generation proceeded without deviations from the normal.
