ABSTRACT
The ethanol-soluble compounds present in the roots of chilcuague (Heliopsis longipes) were obtained with three different extraction methods and analyzed using GC-MS Affinin was isolated using preparative high-pressure liquid chromatography and then analyzed with ultraperformance liquid chromatography-mass spectrometer. Affinin quantification was carried out using high-performance liquid chromatography. Total phenolic compounds were evaluated following the Folin–Ciocalteu method, whereas antioxidant activity was analyzed performing 2,2 Diphenyl-1-picrylhydrazyl assays. Additionally, the bactericidal effect of the extracts was evaluated on Gram-positive and Gram-negative bacteria, as well as on bacteria obtained from buccal microbiota. Finally, genomic DNA was extracted from H. longipes leaves in order to characterize the barcoding sequences trnH-psbA, rbcL, and matK for the proper identification of the plant species. Based on these analyses, the use of H. longipes ethanolic extract was proposed as a coactive agent in the treatment of pharyngitis.
