ABSTRACT
Differences in lysis between Gram-positive and Gram-negative bacteria as well as Archaea are pointed out where appropriate. During the preparation of plasmid DNA, lysis should allow for the plasmids to exit the cell without releasing any of the genomic DNA. Mechanical lysis on its own is suitable for a wide range of bacteria and is rapid, but overall, RNA yields are generally lower than when used in conjunction with enzymatic methods. Large-scale lysis of bacterial filter cakes via fragilization followed by heat has been shown to be viable. Because mechanical lysis breaks cells completely all intracellular components, including nucleic acids are released. However, most mechanical methods of lysis, such as the French press or bead beating, are effective and allow archaea to be treated the same as bacteria. The lysis methods discussed in this chapter are the most popular, but not necessarily the sole methods of lysis.
