ABSTRACT
Nanoemulsions are extensively used to encapsulate the bio-active molecules to provide stability and having numerous applications in food and pharmaceutical industry. There are several factors which influence the stability, functionality and the formation properties of nanoemulsion. The morphological characteristics including particle size, and surface properties are one of the important characteristics which influence stability and functionality. Therefore, the main focus of the present chapter is to discuss different imaging techniques viz. transmission electron microscopy (TEM), scanning electron microscopy (SEM) and atomic force microscopy (AFM) for evaluation of nanoemulsions. Further, the fundamentals and applications of advance imaging technique viz. confocal laser scanning microscopy (CLSM) is also discussed in this chapter. The principles, advantages and limitations of each technique are discussed along with their applications for characterization of nanoemulsions containing bio-actives/nutraceuticals.
