ABSTRACT
Vinblastine (VLB) is one of the alkaloids produced in substantial quantity by perennial shrub, Vinca rosea. Vinblastine is widely extracted from natural resources and used in chemotherapy of various types of hematological lymphoma and leukemia. Anticancer activity of VLB has been assigned mainly to its binding affinity to tubulin resulting in inhibition of tubulin oligomerization and cell apoptosis. Several methods have been used to study the binding interactions of VLB with tubulin and other biomolecules. UV spectroscopy, X-ray crystallography, and computational tools have been utilized to study the binding interactions. Herein, the methods available for the investigation of interaction of vinblastine with biomolecules such as proteins and nucleic acids and reasons for its anticancer activity have been discussed.
