ABSTRACT
This chapter describes a surface acoustic wave (SAW) immunosensor for on-site monitoring of airborne house dust mite allergens. To avoid the exposure to airborne HDMs and reduce the chance of sensitization, we have proposed a monitoring system for airborne HDMs. Fabrication of the SAW immunosensor begins with surface modification with a self-assembled monolayer of two mixed molecules. Based on the results demonstrating unique advantages of the SAW immunosensor including high selectivity, reusability and high sensitivity, the SAW immunosensor is a potential sensor for the airborne allergen monitoring system. Concentration-dependent sensor output from 4CN precipitate is observed. Calibration curve summarizing the SAW immunosensor that employs the enzymatic precipitation method. A drastic enhancement of the sensor output is observed for 1 ng/mL of Der f 2 by the enzymatic precipitation method, where no sensor output is obtained by the conventional SAW immunosensor.
