ABSTRACT
Fluorescent molecules have been widely utilized in scientific researches, medical uses and industrial purposes. Amarastelline A and nigakinone contain a common canthin-5,6-dione structure, that is a tautomer of 5-hydroxy-canthin-6-one for the case of nigakinone. The fluorescent peptide 18 could sense its interaction with calmodulin in the presence of calcium ion by fluorescent ratiometric measurement. Our findings indicated the usefulness of natural compounds for the development of fluorescent molecules with novel structures and functions. To apply this function to the study of biomolecule interaction, C5 peptide, that could interact calmodulin, labelled with this fluorophore, was synthesized, and its interaction with calmodulin could be detected by the ratio of the fluorescence intensities at two fluorescent maximum wavelengths. Our findings indicated the usefulness of natural compounds for the development of novel fluorescent molecules, as well as the versatility of the canthin-5,6-dione and 1,5-naphthyridin-2(1H)-one.
