ABSTRACT
We performed evaluation of enzymatic activity of exogenic enzymes of Bacillus subtilis CABI, which are the base of numerous probiotic preparations. The mutagen phosphemidum was used in the experiments in five concentrations: 0.1%, 0.01%, 0.001%, 0.0001%, and 0.00001%. The following enzymatic activities of B. subtilis were determined after treatment with phosphemidum in the mentioned concentrations: amylolytic (EC 3.2.1), protease (EC 3.4.2), lipase (EC 3.1.1.3), xilanase (EC 3.2.1.8), CMC-ase (carboxymetylcellulase) (EC 3.2.1.203), and β-gluconase (EC 3.2.1.4). High fermentative effectiveness was shown at phosphemidum concentrations of 0.0001% and 0.00001%. Dynamics of changes in enzymatic activity with age of the culture was studied. It was shown that with increasing age of the cultures, the activity of the studied enzymes decreases.
