ABSTRACT
The present study reports on the biosynthesis of silver nanoparticles (AgNPs) with the root extract of Trigonella foenum-graecum (TFAgNPs) and its anticancer activity against A498 cancerous cells by apoptosis through mass drug administration (MDA). The characteristic reaction observed by the color change from neutral to visible brown at pH 11.0 was due to SPR. The scanning electron microscope (SEM) analysis indicated that the spherical shape with size between 20 and 40 nm. In the UV–Vis spectral study was maximum absorption peaks were noted at 420 and 430 nm, respectively. The particle size distribution (PSD) of the TFAgNPs using dynamic light scattering showed that their average size is 120 nm. The X-ray diffraction (XRD) patterns indicate that TFAgNPs comprise a high-intensity diffraction peak at 38.18° corresponding to that of the crystalline Ag. The Fourier transform infrared spectroscopy (FTIR) spectra study confirmed that many functional groups are associated in TFAgNPs; they could eliminate the cancerous A498 cells better than the normal PBMCs. TFAgNPs exhibited a dose-dependent decline in cell viability with an increasingly significant in MDA and decrease intracellular. The apoptosis significantly increased in FTAgNPs treated cells and resulted in a significant decline in caspase-8 role with conflicting upturns in caspase-3/-7/-9 roles. Further, the western blots revealed an augmented expression of smac/DIABLO, p53, Bax, and PARP-1. These results suggest that FTAgNPs induce cell death with A498 via mt facilitated key apoptotic program. Further detailed exploration is necessary to promising using of TFAgNPs in other cancer cell therapy trials.
