ABSTRACT
To obtain a commercial production license, industry must demonstrate to the regulatory agencies that only is the product safe and effective, but also that the production process is developed according to high standards of safety, quality. Choice of an expression system is the first and one of the main key aspects for efficient production of a functional and safe protein. Plant cells can be considered as alternative platform for production instead of microbial and animal cells because they are eukaryotic, easily grown in high densities and rarely contaminate the product with endotoxins pathogens. In addition to the production of recombinant proteins using baculoviral vectors, it is also possible to carry out this production in genetically modified insect cells in a stable manner. While expression systems, such as yeasts and bacteria, allow the production of the target protein at high levels in low-cost culture media, mammalian cells need complex, high-cost culture media for the maintenance of growth and subsequent protein expression.
