ABSTRACT
Esterases are hydrolase-class enzymes involved in cleavage catalysis and ester-bond formation. Esterases are part of inter-esterification, intra-esterification, and responses to transesterification. To isolate esterases, several techniques are employed to screen and identify esterases. For microorganisms that produce esterases, several screening and identification procedures are used. The greatest purity of esterase is achieved via several purifying procedures. Esterases’ industrial applications contribute significantly to environmentally friendly nature initiatives, as well as the food and textile industries. The current review focuses on different aspects, namely screening and purification processes for esterase enzyme analyses. The current evaluation also highlights its potential uses in several domains. To examine it in-depth, we strive to provide a vast array of esterase enzyme knowledge. It is critical to note that esterases are less explored enzymes with a low literature survey than lipases, implying that the future scope of the study will be quite beneficial.
