Retention Indices in Reversed-Phase HPLC
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Retention Indices in Reversed-Phase HPLC book
High-performance liquid chromatography (HPLC) is now a widely adopted technique with a multiplicity of applications. Its suitability for quantitative analyses have been confirmed by both interlaboratory and intralaboratory comparisons. This chapter discusses the developments so far in the provision of reference methods for reporting retentions in reversed-phase separations in HPLC and their applications. It focuses on proposals for retention indices analogous to the systems used in gas-liquid chromatography. The chapter describes the suggestions that individual members of sets of standards could be used to determine relative retentions over a range of elution conditions. In HPLC direct experimental retention times or retention volumes are easy to measure but these values are very dependent on the experimental conditions, including the nature of the stationary phase, composition of the mobile phase, flow rate of the mobile phase, internal dimensions of the column and connecting tubing, and column temperature as well as the relative proportions of the stationary and mobile phases.